INDUCTION/DIFFERENTIATION OF PLURIPOTENT STEM CELL INTO INTESTINAL TRACT EPITHELIAL CELL

摘要

The present invention addresses the problem of providing a novel means for preparing a cell having a function closer to that of an intestinal tract epithelial cell occurring in a living body in a simple and highly efficient manner. A pluripotent stem cell can be differentiated/induced into an intestinal tract epithelial cell through the steps of: (1) differentiating the pluripotent stem cell into an intestinal tract stem cell-like cell; and (2) differentiating the intestinal tract stem cell-like cell obtained in step (1) into an intestinal tract epithelial cell-like cell using a combination of an MEK1 inhibitor, a DNA methylation inhibitor, a TGF-β receptor inhibitor, an EGF and an indigestible polysaccharide.