METHODS FOR PREPARING CDNA SAMPLES FOR RNA SEQUENCING, AND CDNA SAMPLES AND USES THEREOF

摘要

The invention relates to methods for preparing cDNA samples for RNA sequencing using random priming oligonucleotides comprising a cell barcode (cID), a unique molecular index (UMI), and a random sequence region, and performing a reverse transcription reaction (RT). The invention also relates to cDNA samples prepared by the methods and uses thereof.