首页>
外国专利>
METHODS OF PREPARING SAMPLES FOR PROTEOMIC ANALYSIS
METHODS OF PREPARING SAMPLES FOR PROTEOMIC ANALYSIS
展开▼
机译:蛋白质组学分析样品的制备方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
Provided herein are methods of preparing a protein sample for proteomic analysis. In exemplary embodiments, the method comprises (a) contacting a blood sample comprising proteins with a protective agent comprising an anticoagulant (AC) and an aldehyde releaser (AR), to obtain a mixture, optionally, wherein the blood sample is added to a blood collection tube (BCT) comprising the protective agent, and (b) isolating a fraction comprising proteins or a source of proteins from the mixture to yield a protein sample or a source of a protein sample, wherein steps of the method are carried out in the absence of exogenous proteolytic enzyme inhibitors, wherein the protein sample is suitable for proteomic analysis. Preferably, the protective agent consists of essentially (i) about 300 g/l to about 700 g/l imidazolidinyl urea; (ii) about 20 g/l to about 60 g/l glycine; and (iii) about 60 g/l to about 100 g/l EDTA; and the the protein sample is analysed via mass spectrometry-based proteomic methods.
展开▼
机译:本文提供了制备用于蛋白质组学分析的蛋白质样品的方法。在示例性实施方案中,该方法包括(a)使包含蛋白质的血液样品与包含抗凝剂(AC)和醛释放剂(AR)的保护剂接触,以获得混合物,任选地,其中将血液样品添加至血液中。包含保护剂的收集管(BCT),以及(b)从混合物中分离出包含蛋白质或蛋白质源的馏分,以产生蛋白质样品或蛋白质样品源,其中该方法的步骤在不存在外源蛋白水解酶抑制剂,其中所述蛋白质样品适合于蛋白质组学分析。优选地,保护剂基本上由(i)约300g / l至约700g / l的咪唑烷基亚基脲组成; (ii)约20 g / l至约60 g / l甘氨酸; (iii)约60 g / l至约100 g / l EDTA;通过基于质谱的蛋白质组学方法分析蛋白质样品。
展开▼