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HUMAN UMBILICAL CORD MESENCHYMAL STEM CELL SHEETS AND METHODS FOR THEIR PRODUCTION
HUMAN UMBILICAL CORD MESENCHYMAL STEM CELL SHEETS AND METHODS FOR THEIR PRODUCTION
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机译:人脐带间充质干细胞片及其生产方法
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摘要
The disclosure provides a human umbilical cord mesenchymal stem cell sheet comprising one or more layers of confluent human umbilical cord mesenchymal stem cells (hUC-MSCs). The disclosure also provides method for producing hUC-MSC sheets comprising culturing hUC- MSCs in culture solution on a temperature-responsive polymer which has been coated onto a substrate surface of a cell culture support, wherein the temperature-responsive polymer has a lower critical solution temperature in water of 0-80°C; adjusting the temperature of the culture solution to below the lower critical solution temperature, whereby the substrate surface is made hydrophilic and adhesion of the cell sheet to the surface is weakened; and detaching the cell sheet from the culture support.
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