NEW-TYPE SINGLE-BASE EDITING TECHNIQUE AND USE THEREOF

摘要

Provided in the present invention are a new-type single-base editing technique and the use thereof. Specifically provided in the present invention is a gene editing enzyme, characterized in that the structure of the gene editing enzyme is as shown in formula I: Z1-L1-Z2-L2-Z3-L3-Z4 (I), wherein Z1 is the amino acid sequence of the adenine deaminase TadA; Z2 is the amino acid sequence of the TadA* enzyme; and Z1 and/or Z2 have/has a mutation corresponding to the F residue at position 147 and/or 148 of the sequence shown in SEQ ID NO:1; Z3 is the coding sequence of Cas9 nuclease; L1, L2 and L3 are each independently an optional connecting peptide sequence; Z4 is a non- or nuclear localization signal element (NLS); and each "-" is independently a peptide bond. Further provided in the present invention is a method for the site-directed editing of single-base genes. In the method of the present invention, the accuracy of DNA editing is high, and an RNA off-target effect can be significantly reduced.