METHOD FOR DETERMINING A REFERENCE TUMOR AGGRESSIVENESS MOLECULAR GRADIENT FOR A PANCREATIC DUCTAL ADENOCARCINOMA

摘要

The present invention relates to a method for determining a reference tumor aggressiveness molecular gradient for at least one pancreatic ductal adenocarcinoma (PDAC) sample, to a method for determining a specific tumor aggressiveness molecular gradient from a plurality of particular PDAC samples, and to methods for determining a tumor aggressiveness of a PDAC sample to be diagnosed. The method for determining a reference tumor aggressiveness molecular gradient comprises: generating a first matrix of transcriptomic profiles from first vectors representing respectively reference transcriptomic profiles of tumor samples of human or animal origin; obtaining, for each reference transcriptomic profile, a tumor aggressiveness histological class; decomposing the first matrix by a matrix factorization method allowing an identification of at least partially decorrelated components; identifying a first component that is the most discriminating relatively to the tumor aggressiveness histological classes associated with the reference transcriptomic profiles; determining the reference tumor aggressiveness molecular gradient as a distribution of projections of the first vectors on the first component.