Live cells well plate Functional well plate system for electro-mechanical stimulation and analysis of live cells

摘要

The present invention relates to a system that can analyze the deformation, contraction force, the change in the number of beats of the cells according to the stimulation while culturing the cells in real time through the functional well plate and incubator capable of electromagnetism through a microscope and laser displacement meter Specifically, cardiac muscle cells cultured on a diaphragm designed with polydimethylsiloxane (PDMS) are used to mature cells through mechanism stimulation and staining of specific proteins in the cells through staining to confirm their distribution and location. The present invention relates to a cell analysis system capable of analyzing the maturation and measuring changes in contractility and heart rate of cardiomyocytes in real time through a laser displacement meter integrated on a microscope. Electromagnetism and analysis system according to the present invention can be measured in real time using the microscope and laser displacement meter of the shape and contraction force of the cell according to the electromagnetism of the cardiac muscle cells and effective in optimizing the stimulation. In addition, the electromagnet pole and analysis system according to the present invention can be composed of a total of three wells, it is possible to analyze the response according to the drug through the cells matured through the electromagnet pole through a microscope and a laser displacement meter. Additional wells can be identified through structural changes.