EXPRESSION VECTOR ELEMENT COMBINATIONS NOVEL PRODUCTION CELL GENERATION METHODS AND THEIR USE FOR THE RECOMBINANT PRODUCTION OF POLYPEPTIDES

摘要

The use of human kidney factor 1 alpha promoter (with Intron A) for transient transfection provides enhanced productivity (in LC-HC-SM configuration), and the use of bovine growth hormone polyA signal sequence makes use of SV40 polyA signal sequence. Provides enhanced productivity compared to, and the addition of HGT to the bGH PolyA signal sequence results in increased productivity in a vector comprising the hCMV promoter, and the vector construct LC (3'-5 ')-HC-SM It is reported herein to provide improved expression. For stable vowels, vowels generated with vectors containing the hEF1α promoter show enhanced productivity in batch analysis, clones generated with the vector containing the hEF1α promoter show a reduced number of low production clones, and the hEF1α promoter It is reported herein that clones generated using the containing vector exhibit higher stability of IgG expression. For single clones, the vector construct downstream of the selection marker (LC-HC-SM) positively affects the productivity of the single clone, while clones generated using vectors containing the bGH polyA signal sequence and hGT are higher. It is reported to have productivity.