SDS-PAGE Identification method of high-molecular weight glutenins using reversed-phased high-performance liquid chromatography and SDS-PAGE

摘要

The present invention relates to a method for identifying polymer glutenine subunits using high performance liquid chromatography and SDS-PAGE. Through the present invention, the conditions for analysis of RP-HPLC have been improved. The separation precision and reliability of the glutenine subunit is increased, so that it can be applied to high quality wheat breeding with improved processing aptitude. The separated polymer glutenine subunit is also highly related to gluten allergy and can be applied in the food and medical field. .