The present invention discloses a colorimetric fluorescent glucose sensor using a carbon dot and an organic dye and a method of manufacturing the same. A solid biosensor according to the present invention is a sensor having a characteristic that a fluorescent color changes in proportion to a glucose concentration. The fluorescent glucose sensor has a similar proportion of fluorescence color change, sensitivity and good selectivity as a solution state. The ratiometric biosensor visually increase the visibility of the glucose sensor compared to simple turn-on or turn-off modes. Thus, a convenient and effective method of detecting glucose enables a wide range of applications of carbon dots to biosensors.
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