How to measure intermolecular and / or intramolecular interactions

摘要

A method of measuring the interaction between a labeled particle and a ligand comprising the following steps: a) providing a sample comprising labeled particles and a ligand in a solution, wherein the labeled particles are dissolved or dispersed in the solution or immobilized on a solid support; b) fluorescently exciting the labeled particles and detecting fluorescence of the excited particles at a predetermined temperature; c) repeating steps (a) and (b) several times at different concentrations of ligands in solution; And d) measuring the interaction between the labeled particle and the ligand based on the ligand concentration dependent fluorescence change of the labeled particle, The labeled particles are labeled with one or more dyes.