How to separate 18 ingredients from traditional herbal composition

摘要

The present invention belongs to the field of quality analysis and control of traditional Chinese medicine, and provides a method of separating 18 components in a traditional Chinese medicine composition, which method comprises: (1) Traditional Chinese medicine composition is made into a whole extract, water, 10% Ethanol and 30% ethanol were eluted in this order to separate the resin, and 30% ethanol eluate was collected to obtain a 30% ethanol extract; (2) adding the 30% ethanol extract to reverse phase silica gel ODS-AQ-HG and separating in a medium pressure separation column to obtain a number of elution drying pastes each numbered; (3) Each numbered elution dry paste was dissolved in 30% methanol as a solvent, and the solution was passed through a 0.45 μm microporous membrane, primary separated by high performance liquid chromatography, and chromatographic peaks at different residence times. Collected, and also purified by high performance liquid chromatography, finally yielding 18 components. The separation method of the present invention enables an in-depth study of chemical components of pharmaceutical compositions.