WNT/SFRP complexes, WNT-containing compositions, WNT-expressing cells, and methods of making, purifying, and using them

摘要

Disclosure of the invention The isolated protein complex comprising Wnt and sFRP; A composition comprising Wnt; Cells overexpressing Wnt and sFRP; A composition comprising cells overexpressing Wnt and cells overexpressing sFRP; Protein complexes, compositions and methods of making cells; And methods of using isolated protein complexes, compositions and cells. The present disclosure further describes a method for forming a complex comprising Wnt and sFRP and a method for separating Wnt. Methods that can be used to purify Wnt without the use of detergents are also described herein.