3 Hydrogel micro-well arrays based on micro-through-hole and method for three dimensional single cell isolation and culture using thereof

摘要

The present invention relates to a micro-penetration-based hydration gel microwell array and a three-dimensional single cell separation and cultivation method using the same.In particular, the present invention is disposed in a non-contact manner on the bottom of a cell culture vessel, and a constant gap between the micro-penetrating membranes A micro-penetrating-based hydration gel microwell array for separation and cultivation of single cells including a micro-penetrating membrane holding the micro-penetrating membrane and a hydration gel filling the micro-penetrating membrane; A method of manufacturing the hydrogel microwell array; Isolation and culture method of single cells using this; And a single cell separation device.