H Method for Detecting Ribonuclease H Activity Using Catalytic Hairpin Assembly

摘要

The present invention relates to a method for detecting ribonuclease H (RNase H) activity using hairpin self-assembly, and more particularly, a method for detecting RNase H activity without a label with high sensitivity through hairpin self-assembly-based signal amplification. It is about. The RNase H activity detection method based on the unlabeled hairpin self-assembly according to the present invention does not require fluorescent and quencher material labeling, and does not cause an error signal due to the low stability of the detection system. And RNase H activity can be detected with high sensitivity, and selection of RNase H inhibitors with potential as a treatment for AIDS (AIDS) is also possible.