首页> 外国专利> N-CNDsnitrogen-doped carbon nanodots Campylobacter jejuni Detection method of Campylobacter jejuni with enhanced detection efficiency using N-CNDs

N-CNDsnitrogen-doped carbon nanodots Campylobacter jejuni Detection method of Campylobacter jejuni with enhanced detection efficiency using N-CNDs

机译:N-CNDs氮掺杂的空肠弯曲菌碳纳米点N-CNDs检测空肠弯曲菌的方法

摘要

The present invention relates to a detection method of Campylobacter jejuni, a method for selectively enriching Campylobacter jejuni, a Campylobacter jejuni selective detection culture medium including carbon nanodots, and a Campylobacter jejuni selective detection culture kit including the culture medium. The detection method comprises the steps of: (a) selectively enriching Campylobacter jejuni in a sample by culturing the sample through irradiating an enrichment culture medium including nitrogen-doped carbon nanodots with a blue LED; and (b) detecting Campylobacter jejuni in a culture liquid including the enriched Campylobacter jejuni. According to the present invention, in the detection method, if a blue LED is irradiated when a sample is cultured in a culture medium including nitrogen-doped carbon nanodots, it has been confirmed that the growth of other bacteria is controlled and selective enrichment by the carbon nanodots is possible for Campylobacter jejuni. Thus, if the detection method is applied to an ELISA method or the like, higher detection efficiency is exhibited, and a detection time is also reduced.
机译:空肠弯曲菌的检测方法,空肠弯曲菌的选择性富集方法,包括碳纳米点的空肠弯曲菌选择性检测培养基,以及包含该培养基的空肠弯曲菌选择性检测培养基。该检测方法包括以下步骤:(a)通过用蓝色LED照射包括氮掺杂的碳纳米点的富集培养基,通过培养样品来选择性地富集空肠弯曲杆菌。 (b)在包含浓缩的空肠弯曲杆菌的培养液中检测空肠弯曲杆菌。根据本发明,在该检测方法中,如果在包含氮掺杂的碳纳米点的培养基中培养样品时照射蓝色LED,则可以确认通过该方法可以控制其他细菌的生长并进行选择性富集。空肠弯曲杆菌可能存在碳纳米点。因此,如果将检测方法应用于ELISA方法等,则显示出更高的检测效率,并且还减少了检测时间。

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