Method for Exosome Staining and Quantification Using Fluorescence Polarization Technique

摘要

The present invention relates to a method for staining and quantifying exosomes using a fluorescence polarization technique, and more specifically, staining exosomes using a lipid membrane fluorescent dye, and then analyzing the fluorescence polarization values to quantify the exosomes. Method, kit for exosome staining and quantification using the method, and information providing method for cancer diagnosis. In the method for staining and quantifying exosomes using the fluorescent polarization technique of the present invention, all reactions are performed in a single tube without washing, and exosomes can be stained and quantified quickly and efficiently without complicated processes. In addition, the method for quantifying exosomes of the present invention successfully quantifies exosomes secreted from cancer cells and normal cells with high precision and reproducibility compared to a commercial kit, and can effectively measure the increased exosomes in cancer cells. It has the effect of performing an early diagnosis quickly and simply.