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Primer set for determining cucumber mosaic virus infection of Capsicum annuum

机译:用于确定辣椒辣椒黄瓜花叶病毒感染的引物组

摘要

The present invention relates to a set of primers for discriminating cucumber mosquitovirus infection of red pepper, specifically, an oligonucleotide primer represented by SEQ ID NO: 1, an oligonucleotide primer represented by SEQ ID NO: 2, an oligonucleotide primer represented by SEQ ID NO: 3 and a sequence It relates to a primer set, a kit for determining the cucumber mosquito virus infection of pepper containing the oligonucleotide primer represented by the number 4, and a method for determining the cucumber mosquito virus infection of pepper using the same. When RT-PCR is performed using the primer set of the present invention, it is possible not only to determine whether the cucumber is infected with cucumber mosaic virus, but also to quickly and accurately determine the high and low pathogen types. Accordingly, it can greatly contribute to the early diagnosis of infection of highly pathogenic cucumber mosaic virus and to prevent the spread of damage, and is also expected to be of great help in developing resistant varieties against highly pathogenic cucumber mosaic virus.
机译:本发明涉及用于区分红辣椒的黄瓜花叶病毒感染的一组引物,具体地讲,由SEQ ID NO:1代表的寡核苷酸引物,由SEQ ID NO:2代表的寡核苷酸引物,由SEQ ID NO:2代表的寡核苷酸引物。 :3和序列涉及引物组,用于确定辣椒的黄瓜蚊虫病毒感染的试剂盒,其包含由数字4表示的寡核苷酸引物,以及使用该试剂盒确定辣椒的黄瓜蚊虫病毒感染的方法。当使用本发明的引物组进行RT-PCR时,不仅可以确定黄瓜是否被黄瓜花叶病毒感染,而且可以快速而准确地确定高和低病原体类型。因此,它可以极大地有助于高致病性黄瓜花叶病毒的感染的早期诊断并防止损害的传播,并且有望在开发针对高致病性黄瓜花叶病毒的抗性品种中有很大的帮助。

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