PHARMACEUTICAL COMPOSITION COMPRISING THE EXTRACTION OF SPENT MUSHROOM SUBSTRATE FOR LENTINUS EDODES AS AN EFFECTIVE COMPONENT FOR PREVENTION OR TREATMENT OF THROMBOSIS AND HEALTH FUNCTIONAL FOOD COMPRISING THE SAME

摘要

The present invention occurs as a by-product when cultivating shiitake mushrooms ( Lentinus edodes ), a pharmaceutical composition for preventing or treating thrombosis containing an extract of a post-harvest medium (spent mushroom substrate, SMS) as an active ingredient and a health functional food More specifically, after inoculation and cultivation of shiitake mushrooms in a medium for artificial cultivation of shiitake mushrooms, after harvesting shiitake mushrooms, hot water extract of the remaining waste medium and its ethyl acetate fraction, butanol fraction and sequential organic solvent fraction The present invention relates to a pharmaceutical composition for preventing or treating/improving thrombosis through strong blood clotting inhibition using water residue as an active ingredient and a health functional food. The pharmaceutical composition for preventing or treating thrombosis of the present invention and the extract of SMS generated as a by-product during artificial cultivation of shiitake mushrooms as an active ingredient of a health functional food is a powerful antithrombosis caused by inhibition of an enzyme related to thrombus production and inhibition of blood clotting factors. At the same time, it exhibits activity, does not show any hemolytic activity against human red blood cells, has excellent thermal stability, and does not exhibit blood coagulation factor inhibition and loss of enzyme inhibitory effects related to thrombus production, even in acidic conditions of pH 2 and plasma, improving blood circulation It is expected that it can be used for the prevention and treatment of thrombosis such as ischemic stroke and hemorrhagic stroke, and the active ingredient is processed into various forms such as extracts, powders, pills, tablets, and can be prepared in a form that can be taken at all times. It is a very useful invention in the pharmaceutical industry and food industry because of its outstanding effect.