IRX1 - IRX1 Knock-out Transgenic Zebrafish Model and Method for Producing Thereof

摘要

The present invention relates to a zebrafish model knock-out of the IRX1 gene and uses thereof, and more specifically, a transformed zebrafish in which the Irx1a and Irx1b genes are knocked out, the method of manufacturing the zebrafish, the Provided are a method for screening a candidate substance for cancer treatment using zebrafish and a method for predicting the therapeutic effect of a cancer treatment agent on cancer. The transformed zebrafish in which Irx1a and Irx1b are knocked out according to the present invention lacks Irx1a and Irx1b functions, resulting in tumors having tumor biological properties very similar to human tumors. Therefore, the zebrafish cancer model of the present invention is an excellent animal model for studying the formation, metastasis, and proliferation of tumors in vivo, and is tailored to patients through efficient screening of cancer treatment agents and prediction of treatment effects of cancer treatment agents against cancer. It can be useful in establishing treatment strategies.