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LAMP New primer set for detection of MERS-coronavirus using LAMP and uses thereof

机译:LAMP用LAMP检测MERS冠状病毒的新引物组及其用途

摘要

The present invention relates to a novel primer set for detecting MERS coronavirus using LAMP and uses thereof, specifically, a primer set for ring-mediated isothermal amplification (LAMP) reaction for amplifying the Nsp1 gene and Orf5; A composition for detection comprising it; Detection kit; And a detection method using the same. New primer set for detecting MERS coronavirus using LAMP according to the present invention; A composition for detection comprising it; Detection sheet; And by using the detection method using the same, it is possible to quickly and easily detect the MERS corona virus without expensive equipment directly in the field. In addition, the sensitivity is also very high, so it can be very useful in the diagnosis of MERS coronavirus infection.
机译:本发明涉及使用LAMP检测MERS冠状病毒的新型引物组及其用途,特别是用于环介导的等温扩增(LAMP)反应以扩增Nsp1基因和Orf5的引物组。用于检测的组合物,其包含:检测试剂盒;并使用相同的检测方法。根据本发明的用于使用LAMP检测MERS冠状病毒的新引物组;用于检测的组合物,其包含:检测表;而且,通过使用使用该方法的检测方法,可以在现场直接使用昂贵的设备而快速且容易地检测出MERS冠状病毒。另外,灵敏度也很高,因此对MERS冠状病毒感染的诊断非常有用。

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