Lignin-degrading enzyme redesign method and re-design using it Lignin-degrading enzyme

摘要

Selecting a binding site for lignin peroxidase (LIP); And inducing the formation of a new ionic bond or a covalent bond at the selected bond site, wherein the selected bond site is an ionic bond or share formed at a bond site of manganese peroxidase (MnP). In an enzyme redesign method that includes one or more of the same bonds as the binding, the step of inducing the formation of the new ionic bond or covalent bond causes a mutation in any one or more amino acids adjacent to the selected binding site to generate a variant. It includes a step of forming, and provides a lignin degrading enzyme redesign method and a redesigned lignin degrading enzyme using the same, wherein structural stability is increased by the new ionic bond or covalent bond.