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D- D-glutamate auxotrophic Escherichia coli and method of producing target product

机译：D-d-谷氨酸营养缺陷型大肠杆菌及其生产方法

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摘要

The present invention relates to a D-glutamate auxotrophic mutant E. coli in which the murI gene is deleted, a method for preparing the same, and a method for producing a target substance using the E. coli. The D-glutamate auxotrophic Escherichia coli of the present invention has few reverting mutants, maintains high plasmid stability and protein productivity, and can effectively produce useful products without the use of antibiotics, and can be applied to clean production of useful products including proteins. Do.

机译：本发明涉及其中缺失了 murI 基因的D-谷氨酸营养缺陷型大肠杆菌，其制备方法以及使用该大肠杆菌生产目标物质的方法。本发明的D-谷氨酸营养缺陷型大肠杆菌具有很少的回复突变体，保持了高质粒稳定性和蛋白质生产率，并且可以在不使用抗生素的情况下有效地生产有用的产品，并且可以用于清洁生产包括蛋白质的有用的产品。做。 展开▼

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公开/公告号KR102166288B1

专利类型

公开/公告日2020-10-15

原文格式PDF

申请/专利权人 한국생명공학연구원;
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申请/专利号KR20190025765

发明设计人 이대희;이승구;김성근;우승균;나유진;
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申请日2019-03-06

分类号C12N15/70;C07K14/245;C12N9/88;C12P21;

国家 KR

入库时间 2022-08-21 11:03:33

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2. 新型的基于营养缺陷型互补的非抗生素筛选的大肠杆菌宿主/载体系统 [P] . 中国专利： CN1196792C . 2005.04.13

3. D- D-glutamate auxotrophic Escherichia coli and method of producing target product [P] . 韩国专利： KR20200107140A . 2020-09-16

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机译： D-谷氨酸营养缺陷型大肠杆菌和使用相同物质生产靶标物质的方法

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