Monoclonal antibody with specificity for the toxin of Corynebacterium diphtheriae, hybridoma cell line producing the same and use thereof

摘要

The present invention is a monoclonal antibody that specifically binds to diphtheria toxin; A hybridoma cell line that produces the monoclonal antibody; A composition for detecting diphtheria toxin comprising the monoclonal antibody; It relates to a diphtheria toxin detection kit and a diphtheria toxin detection method comprising the monoclonal antibody. The monoclonal antibody produced from the hybridoma cell line of the present invention has a specific high binding affinity only to toxins secreted by diphtheria bacteria, but does not react with toxins or other proteins secreted by other bacteria at the same time. Monoclonal antibodies can be usefully used in diagnosing diphtheria disease through detection of diphtheria toxin. In addition, using such characteristics, the monoclonal antibody produced from the hybridoma cell line of the present invention can be usefully used to conduct research and develop related technologies, such as observing the treatment prognosis of diphtheria disease, detecting or quantitative analysis of diphtheria toxin. .