A method of preparing an autoprobiotic based on an anaerobic consortium of bacteria

摘要

FIELD: biotechnology.SUBSTANCE: invention relates to producing an autoprobiotic product of a new generation. Method for producing an autoprobiotic agent based on an anaerobic consortium of indigenous bacteria involves preparation of a faecal extract by resuspending in a phosphate buffer. Supernatant is separated by centrifugation, followed by mixing the supernatant with physiological saline and filtration. One introduces the obtained filtrate into a nutrient medium containing a yeast extract, sodium chloride, glucose monohydrate, thioglycolic acid, casein pancreatic hydrolyzate, L-cysteine, granular agar, gelatine and distilled water in given amounts, into which a faecal suspension is then added in a physiological solution containing vitamin K and glucose, followed by cultivation under anaerobic conditions at temperature of 37 °C for 3–6 days, centrifuged, resuspended residue in stabilizing solution containing 10 % sucrose and 1 % gelatine, to produce desired product.EFFECT: invention allows to expand the range of autoprobiotics.1 cl, 18 dwg, 1 tbl, 7 ex