METHOD FOR LOGICAL CONCLUSION ABOUT TRANSCRIPTION FACTOR ACTIVITY OF SIGNAL TRANSDUCTION PATH IN A SUBJECT

摘要

The present invention relates to a method for inferring activity of a transcription factor of a signal transduction pathway in a subject. The method comprises performing (101) a first staining for detecting the transcription factor in cells of a sample of the subject and performing (102) a second staining for detecting a protein encoded by a target gene of the transcription factor in cells of the sample. The method further comprises quantifying (103) cells of the sample that show both a nuclear presence of the transcription factor and a presence of the target gene-encoded protein based on the first staining and the second staining, and inferring (104) the activity of the transcription factor in the subject based on the quantifying. This allows the presented method to more reliably infer the activity of the transcription factor in the subject.