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SET OF OLIGONUCLEOTIDE PRIMERS AND A FLUORESCENCE-LABELED PROBE FOR IDENTIFYING A WEST NILE VIRUS GENOTYPE 2
SET OF OLIGONUCLEOTIDE PRIMERS AND A FLUORESCENCE-LABELED PROBE FOR IDENTIFYING A WEST NILE VIRUS GENOTYPE 2
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机译:一套用于鉴定西尼罗病毒基因型2的寡核苷酸起始分子和荧光探针
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摘要
FIELD: biotechnology.;SUBSTANCE: invention refers to biotechnology, namely to molecular biology. A set of oligonucleotide primers and a fluorescence-labeled probe for identifying West Nile virus 2 genotype by PCR method, having direct and reverse primer activity, and a "molecular beacon" type probe which provides fluorescence detection of the amplification product with size of 116 base pairs in real time.;EFFECT: invention enables high-specific detection of said virus and can be used in epidemiological surveillance of West Nile fever in a short period of time.;1 cl, 1 dwg, 1 tbl, 3 ex
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机译:领域:生物技术;实质:发明是指生物技术,即分子生物学。具有通过PCR方法鉴定西尼罗河病毒2基因型的寡核苷酸引物和荧光标记探针,具有正向和反向引物活性;“分子信标”型探针可对扩增产物进行荧光检测,大小为116个碱基效果:本发明能够对所述病毒进行高特异性检测,并可在短时间内用于西尼罗河热的流行病学监测。1cl,1 dwg,1 tbl,3 ex
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