首页> 外国专利> METHOD FOR ESTIMATING CROSS-CONTAMINATION OF SAMPLES IN NGS-DATA ON ANALYSIS OF HIGH-POLYMORPHIC LOCI

METHOD FOR ESTIMATING CROSS-CONTAMINATION OF SAMPLES IN NGS-DATA ON ANALYSIS OF HIGH-POLYMORPHIC LOCI

机译：NGS数据中样品交叉污染的估计方法-基于高等位基因分析的数据

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FIELD: medicine.;SUBSTANCE: invention relates to molecular biology and diagnostic medicine. Described is a method for determining contamination of samples with DNA admixture. Using 3 pairs of primers for analysis of polymorphisms with higher discriminating force: D1S1655, D12S381, D12S392. Primers have sequences:; <TableGroup colSep="1" cols="2" rowSep="1"> <Colspec colName="c1" colWidth="21mm"/> <Colspec colName="c2" colWidth="71mm"/> <Tbody> <Row> <entry colSep="1" rowSep="1">D1S1655</entry> <entry colSep="0" rowSep="1">GAGATTCAAGAATCACGGAACCGTGTTTGCAGTGGGGTCAACT</entry> </Row> <Row> <entry colSep="1" rowSep="1">D12S381</entry> <entry colSep="0" rowSep="1">AACAGGATCAATGGATGCATTGGCTTTGTTTCCTGGACTG</entry> </Row> <Row> <entry colSep="1" rowSep="0">D12S392</entry> <entry colSep="0" rowSep="0">AACAGGTGCAATGGATGCATAGCCTCCATATGGACTGAGC</entry> </Row> </Tbody> </TableGroup> </TableFormula> </Table> ;See table.;EFFECT: invention can be used to determine in data of high-efficiency sequencing of signs of cross contamination of samples and/or contamination of samples of extraneous human DNA.;1 cl </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span>技术领域本发明涉及分子生物学和诊断医学。描述了一种确定样品被DNA混合物污染的方法。使用3对引物分析具有较高区分力的多态性：D1S1655，D12S381，D12S392。引物具有序列： <表格> <TableFormula> <标题/> <TableGroup colSep =“ 1” cols =“ 2” rowSep =“ 1”> <Colspec colName =“ c1” colWidth =“ 21mm” /> <Colspec colName =“ c2” colWidth =“ 71mm” /> <身体> <行> <entry colSep =“ 1” rowSep =“ 1”> D1S1655 </ entry> <entry colSep =“ 0” rowSep =“ 1”> GAGATTCAAGAATCACGGAACCGTGTTTGCAGTGGGGTCAACT </ entry> </ Row> <行> <entry colSep =“ 1” rowSep =“ 1”> D12S381 </ entry> <entry colSep =“ 0” rowSep =“ 1”> AACAGGATCAATGGATGCATTGGCTTTGTTTCCTGGACTG </ entry> </ Row> <行> <entry colSep =“ 1” rowSep =“ 0”> D12S392 </ entry> <entry colSep =“ 0” rowSep =“ 0”> AACAGGTGCAATGGATGCATAGCCTCCATATGGACTGAGC </ entry> </ Row> </ Tbody> </ TableGroup> </ TableFormula> </ Table> 效果：本发明可用于在高效测序数据中确定样品交叉污染和/或外源人类DNA样品污染的迹象。1cl </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33">著录项</h2> <div class="getdents"> <ul> <li> <p><span>公开/公告号</span><span>RU2717975C2</span></p> <p><span>专利类型</span><span></span></p> </li> <li> <p><span>公开/公告日</span><span>2020-03-27</span></p> <p><span>原文格式</span><span>PDF</span></p> </li> <li> <div class="apply"> <p><span style="width: 90px;display: inline-block;">申请/专利权人</span><span id="patentee" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=12_13&sertext=OBSHCHESTVO S OGRANICHENNOJ OTVETSTVENNOSTYU ONKODIAGNOSTIKA ATLAS&option=232">OBSHCHESTVO S OGRANICHENNOJ OTVETSTVENNOSTYU ONKODIAGNOSTIKA ATLAS;</a> </span></p> <span class="designer hoverxs" style="display: none;">展开▼</span> </div> <p><span>申请/专利号</span><span style="width: 250px;">RU20180128649</span></p> </li> <li> <div class="apply"> <p><span style="width: 90px;display: inline-block;">发明设计人</span><span id="patentee" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=12_13&sertext=ROZHAVSKAYA EKATERINA FELIKSOVNA 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