METHOD FOR CULTIVATION OF CALLUS CULTURE OF COMMON WORMWOOD (ARTEMISIA VULGARIS L.)

摘要

FIELD: biotechnology.;SUBSTANCE: invention represents a method for producing callus culture of wild-common wormwood (Artemisia vulgaris L.) in in vitro conditions, including sterilization of wormwood seeds with hydrogen peroxide solutions (3 % solution) for 10 minutes, ethyl alcohol (70 % solution) for 1 minute, three times rinsing in sterile distilled water for 5 minutes, placing sterile seeds on a solid nutrient medium without hormones, the following composition, mg: NH₄NO₃ – 33000, KNO₃ – 38000, CaCl₂x2H₂O – 8800, MgSO₄x7H₂O – 7400, KH₂PO₄ – 3400, KI – 166, H₃BO₃ – 1240, MnSO₄x4H₂O – 4460, ZnSO₄x7H₂O – 1720, Na₂MoO₄x2H₂O – 50, CuSO₄x5H₂O – 5, CoCl₂x6H₂O – 5, FeSO₄x7H₂O – 5560, Na-EDTA – 7460, mesoinositol – 100, thiamine – 100, pyridoxine – 100, nicotinic acid – 100, saccharose – 2500, water – 1000 ml, agar – 7000, further placement of leaf explants obtained from seedlings into a nutrient medium of the following composition, mg: NH₄NO₃ – 33000, KNO₃ – 38000, CaCl₂x2H₂O – 8800, MgSO₄x7H₂O – 7400, KH₂PO₄ – 3400, KI – 166, H₃BO₃ – 1240, MnSO₄x4H₂O – 4460, ZnSO₄x7H₂O – 1720, Na₂MoO₄x2H₂O – 50, CuSO₄x5H₂O – 5, CoCl₂x6H₂O – 5, FeSO₄x7H₂O – 5560, Na-EDTA – 7460, mesoinositol – 100, casein hydrolyzate – 500, thiamine – 100, pyridoxine – 100, nicotinic acid – 100, saccharose – 30000, 2,4 dichlorophenoxyacetic acid – 1, 6-benzylaminopurine – 1, naphthylacetic acid – 1, water – 1000 ml, agar – 12000; wherein the cultivation of plants is carried out in the dark at temperature of 26±1 °C, humidity of room is 70±5 %, the subculturing cycle is 3 weeks.;EFFECT: invention allows to produce callus culture of common wormwood (Artemisia vulgaris L.) in in vitro conditions.;1 cl, 3 tbl