METHOD OF PURIFYING VITAMIN K-DEPENDENT PROTEINS, SUCH AS COAGULATION FACTOR VII

摘要

FIELD: biotechnology.;SUBSTANCE: described is a method for production of prion-free factor VII with successive purification steps involving chromatography, characterized in that at least one chromatography step is carried out using a multimodal resin, providing a fraction containing factor VII in an aqueous solution, then brought into contact with multimodal resin at pH 6–9; multimodal resin with bound factor VII is washed with an aqueous washing buffer in order to remove impurities and delay factor VII before eluting factor VII, elution step is carried out with a multimodal resin at pH 6–9 with a buffer containing arginine, and fractions containing factor VII are collected. In one embodiment of the method, the multimodal chromatography step is combined with an affinity chromatography step, where the affinity is provided by a ligand based on the protein expressed in yeast, and the purity of the final product of the affinity chromatographic resin is more than 95 %.;EFFECT: invention extends the range of methods for producing factor VII without prion content.;15 cl, 1 dwg, 8 tbl, 7 ex