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BACTERIAL STRAIN OF BACILLUS SUBTILIS WITH COMPLEX BIOLOGICAL ACTIVITY

机译:具有复杂生物活性的枯草芽孢杆菌的细菌菌株

摘要

FIELD: biotechnology.;SUBSTANCE: invention refers to biotechnology, particularly to preparation of preparations and insecticidal action. Disclosed is a strain of bacteria Bacillus subtilis 26DCryChS (RCAM04928), obtained from natural strain B. subtilis 26D by recombinant transformation with plasmid pDG1662, containing target gene cry1Ia (identification number GenBank X62821.1), coding synthesis of insectotoxic protein Cry1Ia. Obtained recombinant B. subtilis 26DSyChS strain preserved the useful utility properties of the initial B. subtilis 26D strain, obtained high insecticidal activity, as well as higher growth stimulating and antagonistic activity.;EFFECT: bacterial strain can be used in plant growing as a base of a single-component microbiological preparation with complex biological activity stimulating growth of plants, mobilizing phosphates, insecticidal and antagonistic.;1 cl, 1 tbl
机译:技术领域:本发明涉及生物技术,尤其涉及制剂的制备和杀虫作用。公开了一种枯草芽孢杆菌26DCryChS(RCAM04928)菌株,其是通过用质粒pDG1662重组转化而从天然枯草芽孢杆菌26D获得的,该质粒含有靶基因cry1Ia(鉴定号GenBank X62821.1),编码合成的昆虫毒性蛋白Cry1Ia。获得的重组枯草芽孢杆菌26DSyChS菌株保留了最初的枯草芽孢杆菌26D菌株的有用效用特性,获得了很高的杀虫活性,以及​​更高的生长刺激和拮抗活性。效果:该细菌菌株可作为植物的基础生长具有复杂生物活性的单组分微生物制剂的研究,该制剂具有刺激植物的生长,动员磷酸盐,杀虫和拮抗作用。; 1 cl,1 tbl

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