Process for the production of sinus node cells ('cardiac pacemaker cells') from stem cells and process for the purification of sinus node cells generated from stem cells

摘要

Process for the production of sinus node cells ("cardiac pacemaker cells") from stem cells, with the exception of human embryonic stem cells, in whichi. at least one sinus node cell-producing polynucleotide or at least one protein encoded by this at least one sinus node cell-producing polynucleotide is introduced into stem cells; ii the resulting stem cells in the presence of the at least one sinus node cell-producing polynucleotide or the at least one protein are differentiated into sinus node cells, the at least a polynucleotide generating sinus node cells comprises a nucleic acid sequence which is selected from the group consisting ofa) nucleic acid sequence selected from the group consisting of Gm20685, Gm21989, 1700027A07Rik, 4930448N21 Rik, Acadvl, Actn2, Adm, Adprhl1k, Ak1, Afap1l , Amfr, Angptl2, Ank, Ankrd1, Ap1m1, Art3, Atp2a2, Atp5a1, BC090627, Chkb, Cpt1b, C230088H06Rik, C630028M04Rik, Cap2, Casq1, Casq2, Cav3, Cdh13, Cfl2, Cn1, Cn2, Ch9 , Ctgf, D430001F17Rik, Def8, Dhrs13os, Dkk3, Dlst, Dnaja4, Dpysl2, Dusp3, Ech1, Ehd4, Eno3, Eral1, Etfd h, Fads3, Fam134b, Fam54b, Fhl1, Foxs1, Fuca2, Gal, Gbas, Gfpt2, Gja1, Gm12940, Gm13938, Gm14812, Gm15418, Gm15494, Gm2990, Got1, Gpx3, Gtf2h3, Gygox, Hadf, Herg, Hadf, Her Hspala, Hspb7, Htra1, Idh3a, Immt, Kars, Kctd20, Lbh, Ldb3, Lmod2, Lpl, Lrrc10, Mb, Meg3, Mest, Mllt11, Mllt3, Mybpc3, Mybphl, Myh6, Myl3, Mylkoz, Myom1, Myom1 Ndrg2, Ndufs1, Ndufv1, Nexn, Nppa, Nppb, Nrg1, Ogdh, Pam, Pcsk2os2, Pdlim5, Pet112l, Phyh, Pkp2, Pln, Pmpcb, Popdc2, Ppp1cb, Ppp1r14c, Ppp1r3g3, Ppp1r3 Rcan1, Rdh13, RP23-149A5.3, RP23-52N2.4, RP24-425N5.7 (AC109204.21), Ryr2, Scrn2, Sdc4, Sdha, Sdhd, Sh3kbp1, Slc16a1, Slc38a4, Slc8a1, Slco3apn, Snap , Snurf, Sorbs2, Spcs3, Sprr1a, Srl, Supt6h, Synpo2l, Tbrg4, Tecrl, Tgfb2, Tgm2, Tmem109, Tmem38a, Tmod1, Tnnt3, Tom1l1, Trdn, Trim55, Ttn, Ugp2, Uqcrcp1, Vdacirc1, Vdac17, and b) nucleic acid sequence which has at least 70% sequence identity to a nucleic acid sequence of group a c) Nucleic acid sequence which hybridizes to a nucleic acid sequence according to a) or b) under stringent conditions.