Genetically-modified bacteria and uses thereof

摘要

A genetically-modified bacterium, for example of the class Actinobacteria, and the use ofsuch a bacterium in the bioconversion of a steroidal substrate into a steroidal product of interest is disclosed. The bacterium is blocked in the steroid metabolism pathway prior to the degradation of the polycyclic ring system, and at the steroid side chain degradation pathway. In a preferred embodiment the genetic modification comprises inactivation of the genes kshA1, kshA2, kshA3, kshA4 and kshA5.