A device and its method of use for sorting motile from non-motile cells, i.e. spermatozoa is disclosed wherein the device comprises a chamber 4, an inlet 5 and an outlet 6 in fluid communication with the chamber 4, and a plurality of discrete barriers 8 disposed in the chamber wherein each discrete barrier comprises at least one wall 15, 16 and at least one acute angled edge 17 orientated towards the outlet 5. Preferably the discrete barriers 8 are arranged as a periodic array (figures 5, 6) and the barriers may be crescent shaped (figure 4) or have a wall in the shape of an arc 14. An intrauterine insemination kit comprising the device and a method for its use are disclosed.
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