Pneumatic printing system in living cells and printing method of using the same

摘要

The pneumatic printing system for living cells according to the present invention includes a solution supply unit 100 for supplying a solution in which living cells are dispersed; A printing head part 200 communicating with the solution supply part 100 and discharging droplets; And a driving unit 300 applying negative pressure and positive pressure to the solution chamber 231; Including, The printing head part 200 includes a top plate 210 with a through hole 211 formed to apply negative pressure and positive pressure generated from the driving part 300 to the flexible membrane 220, and negative pressure and positive pressure generated from the driving part 300. Deformed by the flexible membrane 220 to open or close the injection port 231A, the solution chamber 231 receiving the solution containing the biological cells supplied from the solution supply unit 100 through the injection port 231A, the solution The chamber 231 includes a lower plate 230 formed with a nozzle 233 for discharging droplets by receiving a solution through a channel 232, The driving unit 300 in the pneumatic printing system of living cells, characterized in that it comprises a solenoid valve 310 to apply a negative pressure and a positive pressure to the solution chamber 231, In order to control the magnitude of the surface tension of the solution supplied to the solution chamber 231, a solution containing a surfactant is supplied to the solution supply unit 100, The surface tension of the solution is characterized in that 30 to 45 mN / m, Adjust the maximum inlet pressure of the solution to function as a valve by the capillary force at the inlet of the nozzle 233, The maximum inlet pressure of the solution is characterized in that 0.5 to 1.5 kPa, It may be configured by applying a positive pressure of 25 to 100 kPa to the solution chamber 231 to improve the dispersibility and cell proliferation of the living cells present on the dispensed surface after the droplet discharge.