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Nanopore-based determination of protein charge, sharp, volume, rotational diffusion coefficient, and dipole moment
Nanopore-based determination of protein charge, sharp, volume, rotational diffusion coefficient, and dipole moment
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摘要
Physical parameters of macromolecules are determined by measuring electrical current I over time for translocation events as the macromolecules in solution move between two liquid compartments that are separated by and fluidically coupled through a synthetic nanopore. Values of charge, volume, shape, rotational diffusion coefficient, and dipole moment are derived from the measurements.
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