METHOD FOR HIGH-THROUGHPUT DETECTION OF DIFFERENTIAL EXPRESSION OF PLANT CIRCRNA ALLELIC LOCI

摘要

The invention relates to the technical field of gene expression detection, and provides methods for high-throughput detection of differential expression of plant circRNA allelic loci. The method comprises the following steps: 1) extracting total RNA of a plant sample, and constructing a strand-specific library; 2) performing paired-end sequencing on the strand-specific library with Illumina HiSeq; 3) screening circRNAs data from raw sequencing data; 4) extracting reverse splicing reads at a circRNAs looping position to form the circRNAs data; 5) performing single nucleotide variation detection on the reverse splicing reads; and 6) collecting statistics about the number of reads of different genotypes, which are aligned to SNP loci, in the reverse splicing reads, to align the proportion of the number of reads of different genotypes to the proportion of expression quantities of different genotypes. The methods can accurately detect differential expression of circRNA allelic loci with high throughput.