Method for identifying post-transcriptional modification site at position 5 of uridine or cytidine in RNA

摘要

The present invention relates to a method for identifying a post-transcriptional modification site at position 5 of uridine or cytidine in RNA. [Solution] The method of identifying the post-transcriptional modification site at the 5-position of uridine or cytidine in RNA of the present invention includes (1) mammalian cells lacking or inhibiting the neosynthetic function of uridine monophosphate or cytidine monophosphate. Culturing in a medium containing uridine or cytidine labeled at the 5-position, (2) obtaining the target RNA from the cultured cells obtained in (1), (3) performing mass spectrometry of the target RNA, and (4) By comparing the mass spectrometric spectrum of (3) with the mass spectrometric spectrum in the case where no post-transcriptional modification site at the 5-position of uridine or cytidine in RNA is generated, the 5 Position of post-transcriptional modification site, Including that. [Selection diagram] None