System and method for detecting genetic structure variation using integrated electrophoresis DNA purification

摘要

The electrophoresis cassette can include a sample well, a gel column comprising a separate gel, and an eluting module disposed adjacent to the gel column.The sample may be provided to an electrophoresis cassette, and the high molecular weight DNA may be separated from the sample.A single copy DNA sequence is cleaved at both sides of the repetitive region of the DNA sequence to produce a cleaved sample and subsequently fractionated using gel electrophoresis.The DNA fraction may be separated from the continuous section of the separated gel, and the PCR assay may be performed for the DNA fraction to detect a single copy sequence in the DNA fraction, wherein the single copy sequence comprises an iterative extension sequence.The DNA fractions of the subject may be electrophoretically eluted to form multiple eluting modules.The size of the DNA fraction having an iterative extension sequence may be determined.It is also determined whether the size exceeds the normal repetitive size range.