Therapeutic composition and method by bacterial Mini cells for targeted delivery of bioactive molecules based on antibodies and FC containing targeted molecules

摘要

PROBLEM TO BE SOLVED: To provide the use of bacterial minicells as targeted delivery agents in vivo and in vitro, and to provide genetically engineered eubacterial minicells designed to preferentially target and deliver therapeutically relevant agents using a minicell surface coupling molecule capable of binding and displaying antibodies or other Fc-containing targeting moiety fusions and conjugates.SOLUTION: According to the present invention, a fully intact bacterial minicell comprises: (i) an Fc binding portion of Protein G or an Fc binding portion of Protein A displayed on the surface of the minicell; (ii) one or more bioactive molecules; and (iii) one or more Fc-containing targeting molecules bound to the Fc binding portion. Here, the one or more Fc-containing targeting molecules recognize a eukaryotic antigen.SELECTED DRAWING: Figure 7