Combinational use of mechanical manipulation and programin derivatives to increase Oct4, Sox2, Nanog or c-Myc expression in fibroblasts

摘要

The present invention provides methods and compositions for inducing pluripotency in differentiated mammalian cells. In particular, the methods include mechanically aggregating the cells into discrete masses or embryoid-like bodies and treated them with a small molecule compound. Provided herein are the compositions of the compounds which are derived from programin (e.g., reversine).