Nucleic acid analysis method, nucleic acid analysis program, and device for library preparation

摘要

PROBLEM TO BE SOLVED: To provide a nucleic acid analysis method which enables simple and highly accurate analysis even when the number of nucleic acids to be analyzed is extremely small. SOLUTION: A library preparing step for preparing a library containing a standard nucleic acid having a specific copy number and a nucleic acid to be analyzed in the same system, and creating calibration curve data based on the copy number of the standard nucleic acid having a specific copy number Nucleic acid analysis including a calibration curve data creating step, and a nucleic acid analysis step of specifying the number of base sequences of the analysis target nucleic acid using the calibration curve data while specifying the base sequence of the analysis target nucleic acid Is the way. [Selection diagram] Fig. 26