PROCÉDÉ POUR DÉTERMINER LA LUMINOSITÉ GLOBALE D'AU MOINS UN OBJET DANS UNE IMAGE NUMÉRIQUE

摘要

The present invention relates to a method and a system for the overall brightness of at least one object in a digital image. In particular, the method for determining the overall brightness of at least one object in a digital image, the at least one object being configured to receive molecules comprising genetic information, the method comprising the steps of: generating an area A comprising k sample points, the k points being distributed in the area A according to a predetermined distribution; aligning the center position of the area A to the center position of the at least one object in the digital image; extracting the intensity value at each of the k sample points; and calculating the central tendency of the extracted intensity values at the respective k sample points.