Regeneration of functional neurons for the treatment of nerve damage caused by disruption of blood flow

摘要

A method of treating the effects of disruption of normal blood flow in the CNS of an individual in need thereof according to an aspect of the invention, wherein a therapeutically effective dose of exogenous NeuroD1 is provided in the area where normal blood flow is disrupted. Is provided. 1) a recombinant adeno-associated adenovirus expression vector containing a glial cell-specific promoter operably linked to a nucleic acid encoding a site-specific recombinase, and 2) operably linked to a nucleic acid encoding NeuroD1 A composition comprising a recombinant adeno-associated adenovirus expression vector containing a ubiquitous promoter, wherein the nucleic acid encoding NeuroD1 is inverted and flanked by two sets of site-specific recombinase recognition sites, whereby the recombinase A composition is provided in which the action irreversibly reverses the nucleic acid encoding NeuroD1 such that NeuroD1 is expressed in mammalian cells. [Selection diagram] Figure 1A