PRÉPARATION DE BANQUES DE VARIANTS DE PROTÉINES S'EXPRIMANT DANS DES CELLULES EUCARYOTES ET LEUR UTILISATION EN VUE DE LA SÉLECTION DE MOLÉCULES DE LIAISON

摘要

The invention relates to methods of producing eukaryotic cell libraries encoding a repertoire of binding molecules ("binders"), wherein the methods use a site-specific nuclease for targeted cleavage of cellular DNA to enhance integration of binder genes through endogenous cellular repair mechanisms. Populations of eukaryotic cells are produced in which a repertoire of genes encoding binders are integrated into a desired locus in cellular DNA (e.g., a genomic locus) allowing expression of the encoded binding molecule, thereby creating a population of cells expressing different binders.