PROCÉDÉ DE DÉTERMINATION DE LA SENSIBILITÉ À L'INSULINE TISSULAIRE, PROCÉDÉ D'IDENTIFICATION D'UNE RÉSISTANCE À L'INSULINE ET/OU DE DÉTERMINATION D'UNE PRÉDISPOSITION À UN TROUBLE LIÉ À CELLE-CI, UTILISATION D'UN INDICE DE SENSIBILITÉ À L'INSULINE, KITS DE DIAGNOSTIC ET LEURS UTILISATIONS

摘要

The subject matter of the present invention relates to a method for determining tissue insulin sensitivity in a human subject, consisting in that RNA is isolated from a whole blood sample drawn from the human subject and the expression of the following genes is measured: KLF9, PEMT and EGR1; and on the basis of obtained results of measurements an insulin sensitivity index is determined. The subject matter of the present invention also relates to a method for identifying insulin resistance and/or determining a predisposition for a disorder connected with insulin resistance in a human subject, consisting in that RNA is isolated from a whole blood sample drawn from the human subject and the expression of the following genes is measured: KLF9, PEMT and EGR1; on the basis of obtained results of measurements an insulin sensitivity index is determined and on the basis of the obtained result of the insulin sensitivity index insulin resistance is identified and a predisposition for a disorder connected with insulin resistance is determined. The subject matter of the present invention also relates to an insulin sensitivity index for use as a diagnostic biomarker, for use as a biomarker of insulin resistance, for use as a prognostic biomarker of a disorder connected with insulin resistance and for use for determining a predisposition for a disorder connected with insulin resistance. The subject matter of the invention also relates to the use of such an insulin sensitivity index for monitoring tissue insulin sensitivity, in particular in the course of a disorder and/or a disease connected with insulin resistance, in particular type 2 diabetes. The subject matter of the present invention further relates to diagnostic kits for carrying out such methods and the use thereof for the prognosis, diagnosis, including early diagnosis, and/or therapy monitoring of a disorder connected with insulin resistance.