VERFAHREN ZUR HERSTELLUNG EINES ZWEIPHASEN-NÄHRMEDIUMS FÜR BAKTERIEN

摘要

The present invention relates to a method for preparing a two-phase culture medium (1) packaged in a tube (2) intended for culturing bacteria aerobically, comprising the following consecutive steps: a.1) pouring into the bottom of the tube a first aqueous medium capable of being frozen or gelled, and tilting said tube according to a predetermined inclination with respect to the vertical; and a.2) freezing or gelling said first medium until forming a first solid layer so that the upper surface of the first layer has a predetermined inclination (α2) with respect to the axis (XX') of said tube; and b.1) pouring into the tube a second aqueous medium which is a culture medium capable of forming a solid culture medium, and tilting said tube according to a predetermined inclination (α1) of the longitudinal axis (XX') thereof with respect to the vertical so as to form a second layer of said second medium above said first layer; and b.2) gelling said medium until forming a second layer of said gelled solid culture medium.