ANALYSE DE MESURE ENZYMATIQUE UTILISANT UN SUBSTRAT MODIFIÉ COMPRENANT UN SUBSTRAT FIXÉ SUR UNE MACROMOLÉCULE PAR L'INTERMÉDIAIRE D'UN ESPACEUR

摘要

The invention provides novel reagents and methodologies for detecting free versus bound compounds. It is particularly useful to detect thrombin when it is not bound to A2M in the presence of thrombin bound to A2M by using a modified substrate that is sterically hindered from reacting with the bound thrombin.