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Cytogenetic characterization of Hydrangea involucrata Sieb. and H. aspera D. Don complex (Hydrangeaceae): genetic, evolutional, and taxonomic implications

机译:绣球绣球花的细胞遗传学表征和H. aspera D. Don复合体(绣球花科):遗传,进化和分类学含义

摘要

The subsection Asperae of genus Hydrangea L. (Hydrangeaceae) has been investigated for three reasons: several ambiguous classifications concerning Hydrangea aspera have been published, unexpected differences in genome size among seven accessions have been reported Cerbah et al. (Theor Appl Genet 103:45–51, 2001), and two atypical chromosome numbers (2n = 30 for Hydrangea involucrata and 2n = 34 for H. aspera) have been found when all other species of the genus present 2n = 36. Therefore, these two species and four subspecies of Hydrangea in all 29 accessions were analyzed for their genome size, chromosome number, and karyotype features. This investigation includes flow cytometric measurements of nuclear DNA content and bases composition (GC%), fluorochrome banding for detection of GC- and AT-rich DNA regions, and fluorescent in situ hybridisation (FISH) for chromosome mapping of 5 S and 18 S-5.8 S-26 S rDNA genes. In the H. aspera complex, the genome size ranged from 2.98 (subsp. sargentiana) to 4.67 pg/2C (subsp. aspera), an exceptional intraspecific variation of 1.57-fold. The mean base composition was 40.5% GC. Our report establishes the first karyotype for the species H. involucrata, and for the subspecies of H. aspera which indeed present different formulae, offering an element of discrimination. FISH and fluorochrome banding revealed the important differentiation between these two species (H. involucrata and H. aspera) and among four subspecies of the H. aspera complex. Our results are in agreement with the Chinese classification that places the groups Kawakami and Villosa as two different species: Hydrangea villosa Rehder and Hydrangea kawakami Hayata. This knowledge can contribute to effective germplasm management and horticultural use.
机译:绣球属(Hydrangeaceae)属Asperae子的研究基于以下三个原因:已公开了关于八仙花属的几种模糊分类,已经报道了七个种质之间基因组大小的意想不到的差异Cerbah等。 (Theor Appl Genet 103:45–51,2001),并且当所有其他属都都存在2n = 36时,发现了两个非典型染色体数(绣球绣球为2n = 30,H。aspera为2n = 34)。 ,分析了所有29个种的绣球花的这两个物种和四个亚种的基因组大小,染色体数目和核型特征。这项研究包括对核DNA含量和碱基组成(GC%)的流式细胞仪测量,用于检测富含GC和AT的DNA区域的荧光染料带以及用于5S和18S-染色体作图的荧光原位杂交(FISH) 5.8 S-26 S rDNA基因在H. aspera复合物中,基因组大小范围从2.98(亚种sargentiana)到4.67pg / 2C(亚种aspera),种内变异为1.57倍。平均碱组成为40.5%GC。我们的报告确定了H. involucrata物种和H. aspera亚种的第一个核型,这些亚种确实具有不同的分子式,从而提供了歧视的元素。 FISH和荧光色素谱带揭示了这两个物种(H. involucrata和H. aspera)之间以及H. aspera复合体的四个亚种之间的重要区别。我们的结果与中国分类一致,川上和紫藤属于两个不同的物种:绣球八仙花(Hydrangea villosa Rehder)和绣球八仙花(Hydrangea kawakami Hayata)。这些知识有助于有效的种质管理和园艺使用。

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