Multiple Antigen Detection Assays and Reagents.

摘要

Provided herein are multiple antigen detection (MAD) assays and reagents for performing such assays. MAD assay embodiments are useful for identifying proximal antigens in a sample (e.g. antigens existing in a complex) and are performed by contacting a sample with two or more hybrids, where each hybrid comprises a nucleic acid and a binding partner that specifically binds to an antigen. In these embodiments, the hybrid nucleic acids hybridize to one another when the hybrids bind to proximal antigens, and the hybridization product then is detected, often by a polymerase chain reaction (PCR) process or a nucleic acid binding agent comprising a detectable label. MAD assays and reagents are applicable to antigen detection in situ, chromatin immuno-precipitation and genomic sequencing, detection of proximal intramolecular regions in an antigen, detecting antigen multimerization, diagnostics and therapeutics. Also provided are kits for conducting MAD applications.